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Institute for Quantum Life Science

Single Cell Radiation Biology Team

Update：2023年4月1日更新
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シングルセルタイトル

Project Goals:

シングルセル図​Our goal is to understand the survival strategies of cells and life against radiation by applying advanced quantum technologies. Advanced microbeam technology will be the main technology, which enables ones to precisely "target and shoot" individual cells and also limiting damage to cell nuclei and organelles such as mitochondria in cytoplasm to investigate the defensive intra- and inter-cellular response. In addition, we will be integrating multidiscipline advanced technologies, such as genome editing, live cell imaging, radiation measurement, and nano-quantum sensor, to analyze and evaluate underlying mechanism of radiation induced damages and the cellular responses.

 

Team Leader, Teruaki Konishi

Members (SCRB Gr., iQLS-QST)

Teruaki Konishi   (Team Leader)

Takeshi Yasuda   (Principal Researcher)

Miho Noguchi (Principal Researcher)

Tomoko Yanaka（Assistant）

Nanase Araragi    (Secretary)

Co-affiliated members (QST)

Satoshi Kodaira                 

Shino Homma-Takeda                 

Akihiro Uehara

Masakazu Oikawa                

Cuihua Liu                        

Tomoo Funayama              

Masahiro Kikuchi

Collaborative Researchers

Satoshi Ishii (Associate Prof., Tokyo Denki Univ)

Ryo Ogawara (Assist. Prof, Kyoto University)

Narongchai Autsavapromporn (Assist. Prof., Chiang Mai Univ, Thailand)

Ariyaphong Wongnoppavich (Assist. Prof., Chiang Mai Univ, Thailand)

Nahathai Dukaew (PD., Chiang Mai Univ, Thailand)

Tengku Ahbrizal Tengku Ahmad (Senior Officer, Malaysian Nuclear Agency, Malaysia)

Jun Wang (Prof., Hefei, Chinese Academy of Science, China PR)

Guangming Zhou (Prof. Soochow Univ., China PR)

Chunlin Shao (Prof., Fudan Univ., China PR)

Gabriel O. Sawakuchi (Associate Prof., The Univ. of Texas MD Anderson Cancer Center, USA)

Publications

Group members in bold

2021

  1. D. Flint, S. Bright, C. Mcfadden, T. Konishi, D. Ohsawa, B. Turner, S. H. Lin, D. R. Grosshans, H. Chiu, P. Sumazin, S. F. Shaitelman, G. O. Sawakuchi*, Cell lines of the same anatomic site and histologic type show large variability in intrinsic radiosensitivity and relative biological effectiveness to protons and carbon ions. Medical Physics, (in press)
  2. T. Kusumoto*, R. Ogawara, K. Igawa, K. Baba, T. Konishi, Y. Furusawa, S. Kodaira, Scaling Parameter of the Lethal Effect of Mammalian Cells based on Radiation-Induced OH Radicals: Effectiveness of Direct Action in Radiation Therapy. J Radiat Res, 62 (2021) 86-93.
  3. H. Pan, Y. Song, H. Zhang, Y. Bai, T Konishi, A. Kobayashi, C. Shao*, Y. Pan*, Radiation Engenders Converse Migration and Invasion in colorectal cancer cells through opposite modulation of ANXA2/AKT/GSK2β pathway., Am J Cancer Res, 11 (2021) 61-78.
  4. A. Uehara *, M. Oikawa, I. Tanaka, H. Ishihara, S. Homma-Takeda, Quantitative Analyses of Urinary Uranium by µ-PIXE, Minerals, 11 (2021) 147.
  5. S. Homma-Takeda*, H. Fujishiro, I. Tanaka, H. Yakumaru, K. Ayama, A. Uehara, M. Oikawa, S. Himeno, H. Ishihara, Single-Cell Imaging for Studies of Renal Uranium Transport and Intracellular Behavior, Minerals, 11 (2021) 191.
  6. T. Yasuda*, K. Takizawa, A. Ui, M. Hama, W. Kagawa, K. Sugasawa, K. Tajima, Human SIRT2 and SIRT3 deacetylases function in DNA homologous recombinational repair. Genes Cells, (2021), https://doi.org/10.1111/gtc.12842.

2020

  1. T. Kusumoto, H. Kitamura, T. Konishi, S. Hojo, S. Kodaira*, Significant Changes in Yields of 7-Hydroxy-Coumain-3-Carboxylic Acid Produced under the FLASH Radiotherapy Condition, RSC Advances, 10(2020)38709
  2. T. Konishi*, D. Yoshihara, M. Maeda, D. Ohsawa, N. Usami, K. Hieda, Enhanced Cell Inactivation and Double-Strand Break Induction in V79 Chinese Hamster Cells by Monochromatic X-rays at Phosphorus K-Shell Absorption Peak, Quantum Beam Science, 4 (2020)38.
  3. J. Wang, A. Kobayashi, D. Ohsawa, M. Oikawa, T. Konishi*, Cytoplasmic Radiation Induced Radio-Adaptive Response in Human Lung Fibroblast WI-38 Cells, Radiat Res, 194 (2020) 288-297.
  4. M. Suzuki*, Y. Uchihori, H. Kitamura, M. Oikawa, T. Konishi, Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human Fibroblasts, Life (Basel), 10 (2020).
  5. L. Zhu, Q. Chen, L. Zhang, S. Hu, W. Zheng, C. Wang, Y. Bai, Y. Pan, T. Konishi, J. Guan, C. Shao*, CLIC4 regulates radioresistance of nasopharyngeal carcinoma by iNOS after gamma-rays but not carbon ions irradiation, Am J Cancer Res, 10 (2020) 1400-1415.
  6. R. Ogawara*, T. Kusumoto, A. Kobayashi, T. Konishi, T. Hamano, S. Kodaira, Discrimination of hydroxyl radical yields due to thermal neutrons, fast neutrons, and gamma rays in accelerator-based neutron fields, Radiat Phys Chem, 173 (2020).
  7. R. Ogawara*, T. Kusumoto, T. Konishi, T. Hamano, S. Kodaira, Polyethylene moderator optimized for increasing thermal neutron flux in the NASBEE accelerator-based neutron field, Radiat Meas, 137 (2020).
  8. S. Ishii*, J. Toki, M. Oikawa, D. Ohsawa, T. Konishi, Electrical responses of a carbon nanotube thin-film transistor to MeV proton irradiation in air, Radiation Effects and Defects in Solids, 175 (2020) 440-449.
  9. C. Dong, W. Tu, M. He, J. Fu, A. Kobayashi, T. Konishi*, C. Shao*, Role of Endoplasmic Reticulum and Mitochondrion in Proton Microbeam Radiation-Induced Bystander Effect, Radiat Res, 193 (2020) 63-72.
  10. N. Dukaew, T. Konishi, K. Chairatvit, N. Autsavapromporn, N. Soonthornchareonnon, A. Wongnoppavich*, Enhancement of Radiosensitivity by Eurycomalactone in Human NSCLC Cells Through G(2)/M Cell Cycle Arrest and Delayed DNA Double-Strand Break Repair, Oncol Res, 28 (2020) 161-175.
  11. R. Ogawara*, T. Kusumoto, T. Konishi, T. Hamano, S. Kodaira, Detection of alpha and 7Li particles from 10B(n, α)7Li reactions using a combination of CR-39 nuclear track detector and potassium hydroxide-ethanol-water solution in accelerator-based neutron fields, Nucl. Instrum. Methods Phys. Res. B., 467 (2020) 9-12.
  12. T. Kusumoto*, R. Barillon, S. Okada, T. Yamauchi, S. Kodaira, Improved criterion of the mechanism for forming latent tracks in poly(allyl diglycol carbonate) based on the number of interactions induced by secondary electrons, Radiat. Meas., 138 (2020) 106445.
  13. T. Kusumoto*, R. Ogawara, N. Ludwig, Q. Raffy, S. Kodaira, Water radiolysis with thermal neutrons, fast neutrons and contamination γ rays in the accelerator based thermal neutron field: Time dependence of hydroxyl radical yields, Radiat. Phys. Chem., 174 (2020) 108978.
  14. T. Kusumoto*, S. Okada, H. Kurashige, K. Kobayashi, M. Fromm, Q, Raffy, N. Ludwig, M. Kanasaki, K. Oda, Y. Honda, S. Tojo, J-E. Groetz, R. Ogawara, S. Kodaira, R. Barillon, T. Yamauchi, Evidence for a critical dose above which damage to carbonate ester bonds in PADC appear after gamma ray and ultra soft X-ray exposures, Radiat. Phys. Chem., 170 (2020) 108628.
  15. S. Kodaira*, T. Kusumoto, H. Kitamura, Y. Yanagida, Y. Koguchi, Characteristics of fluorescent nuclear track detection with Ag+-activated phosphate glas, Radiat. Meas., 132 (2020) 106252.
  16. T. Kusumoto*, Y. Matsuya, K. Baba, R. Ogawara, M.S. Akselrod, J. Harrison, V. Fomenko, T. Kai, M. Ishikawa, S. Hasegawa, S. Kodaira, Verification of dose estimation of Auger electrons emitted from Cu-64 using a combination of FNTD measurements and Monte Carlo simulations, Radiat. Meas., 132 (2020) 106256.
  17. R. Ogawara*, S. Kodaira, M. Suda, T. Hagihara, T. Hamano, Thermal neutron field characteristics in the neutron exposure accelerator system for biological effect experiments (NASBEE) facility, Nucl. Instrum. Meth., A949 (2020) 162724.
  18. S. Homma-Takeda*, A. Uehara, T. Yoshida, C. Numako, O. Sekizawa, K. Nitta, N. Sato, Two-dimensional μXAFS for uranium accumulated in kidney of rats exposed to uranyl acetate, Radiat. Phys. Chem., 175 (2020) 108147.
  19. T. Funayama, T. Sakashita, M. Suzuki, Y. Yokota, N. Miyawaki, H. Kashiwagi, T. Satoh and S. Kurashima, An irradiation device for biological targets using focused microbeams of cyclotron-accelerated heavy ions, Nucl. Instrum. Methods Phys. Res. B., 465 (2020), 101-109
  20. M. Suzuki, Z. Soh, H. Yamashita, T. Tsuji and T. Funayama, Targeted central nervous system irradiation of Caenorhabditis elegans induces a limited effect on motility, Biology, 9 (2020), 289
  21. T. Yasuda, T. Funayama, K. Nagata, D. Li, T. Endo, Q. Jia, M. Suzuki, Y. Ishikawa, H. Mitani and S. Oda, Collimated microbeam reveals that the proportion of non-damaged cells in irradiated blastoderm determines the success of development in medaka (Oryzias latipes) Embryos, Biology, 9 (2020), 447

2019

  1. J. Wang, T. Konishi*, Nuclear factor (erythroid-derived 2)-like 2 antioxidative response mitigates cytoplasmic radiation-induced DNA double-strand breaks, Cancer Sci, 110 (2019) 686-696.
  2. W. Tu, C. Dong, J. Fu, Y. Pan, A. Kobayashi, Y. Furusawa, T. Konishi, C. Shao*, Both irradiated and bystander effects link with DNA repair capacity and the linear energy transfer, Life Sci, 222 (2019) 228-234.
  3. D. Ohsawa*, Y. Furusawa, A. Kobayashi, M. Oikawa, T. Konishi, Analysis of SPICE microbeam size using fluorescent nuclear track detector (FNTD), Nucl Instrum Meth B, 453 (2019) 9-14.
  4. T. Konishi*, S. Kodaira, Y. Itakura, D. Ohsawa, S. Homma-Takeda, Imaging Uranium Distribution on Rat Kidney Sections through Detection of Alpha Tracks Using Cr-39 Plastic Nuclear Track Detector, Radiat Prot Dosimetry, 183 (2019) 242-246.
  5. S. Kodaira*, Y. Morokoshi, H.K. Li, T. Konishi, M. Kurano, S. Hasegawa, Evidence of Local Concentration of alpha-Particles from (211)At-Labeled Antibodies in Liver Metastasis Tissue, J Nucl Med, 60 (2019) 497-501.
  6. A. Kobayashi, N. Autsavapromporn, TAF. Ahmad, M. Oikawa, S. Homma-Takeda, Y. Furusawa, J. Wang, T. Konishi*, Bystander WI-38 Cells Modulate DNA Double-Strand Break Repair in Microbeam-Targeted A549 Cells through Gap Junction Intercellular Communication, Radiat Prot Dosimetry, 183 (2019) 142-146.
  7. W. Hu, L. Zhu, W. Pei, S. Pan, Z. Guo, A. Wu, H. Pei, J. Nie, B. Li, Y. Furusawa, T. Konishi, T.K. Hei, G. Zhou, Overexpression of Ras-Related C3 Botulinum Toxin Substrate 2 Radiosensitizes Melanoma Cells In Vitro and In Vivo, Oxid Med Cell Longev, 2019 (2019) 5254798.
  8. Y. Furusawa*, Y. Matsumoto, R. Hirayama, D. Ohsawa, T. Konishi, Dose-Rate and Cell-Killing Sensitivity of High-Linear Energy Transfer Ion Beam, Radiat Prot Dosimetry, 183 (2019) 219-222.
  9. N. Autsavapromporn*, C. Liu, A. Kobayashi, T. Ahmad, M. Oikawa, N. Dukaew, J. Wang, A. Wongnoppavich, T. Konishi, Emerging Role of Secondary Bystander Effects Induced by Fractionated Proton Microbeam Radiation, Radiat Res, 191 (2019) 211-216.
  10. T. Kusumoto*, Y. Ngono-Ravache, E. Balanzat, C. Galindo, N. Ludwig, Q. Raffy, T, Yamauchi, S. Kodaira, R. Barillon, The role of molecular and radical mobility on the creation of CO2 molecules and OH groups in PADC irradiated with C and O ions, Polym. Degrad. Stab., 164 (2019) 102-108.
  11. S. Kodaira*, H. Kitamura, M. Kurano, H. Kawashima, E.R. Benton, Contribution to dose in healthy tissue from secondary target fragments in therapeutic proton, He and C beams measured with CR-39 plastic nuclear track detectors, Sci. Rep., 9 (2019) 3708.
  12. T. Kusumoto*, M. Sakai, A. Yoshida, T. Kambara, Y. Yanagisawa, S. Kodaira, K. Oda, M. Kanasaki, K. Kuraoka, R. Barillon, T. Yamauchi, An update of radial dose distribution theory for the detection threshold of Kapton as a nuclear track detector irradiated with 345 MeV/u U and other heavy ions, Nucl. Instrum. Meth., B460 (2019) 240-243.
  13. T. Kusumoto*, Y. Mori, M. Kanasaki, K. Oda, S. Kodaira, R. Barillon, T. Yamauchi, Drastic decrease of carbonyl group after the loss of ether in PADC exposed to 222 nm UV photons, Radiat. Phys. Chem., 157 (2019) 60-64.
  14. T. Kusumoto*, T. Yamauchi, R. Barillon, Application of radial electron fluence around ion tracks for the description of track response data of polyethylene terephthalate as a polymeric nuclear track detector, Nucl. Instrum. Meth., B461 (2019) 260–266.
  15. R. Ogawara*, M. Suda, T. Hagiwara, S. Kodaira, T. Hamano, Discrimination method for gamma ray doses in neutron fields using an ionization chamber with attenuation filters, Radiat. Prot. Dosim., 183 (2019) 280-2841.
  16. T. Kusumoto*, R. Ogawara, Radiation chemical yield of hydroxyl radicals for accelerator-based boron neutron capture therapy: Dose assessment of 10B(n,α)7Li reaction using coumarin-3-carboxilic solution, Radiat. Res., 191 (2019) 460-465.
  17. S. Homma-Takeda*, C. Numako, K. Kitahara, T. Yoshida, M. Oikawa, Y. Terada, T. Kokubo, Y. Shimada, Phosphorus localization and its involvement in formation of concentrated uranium in the proximal tubules in the kidneys of rats exposed to uranyl acetate, Int. J. Mol. Sci., 20 (2019) 4677-4687.
  18. K. Tajima*, K. Takizawa, T. Yasuda, R. Tsumanuma, S. Okuyama, H. Kumagai, E. Omoto, W. Okumura, Characterization of reemergent anti-B red blood cell antibodies in a patient with recurrent acute myeloid leukemia with ABO-incompatible allogeneic peripheral blood stem cell transplantation. Transfusion, 59 (2019) 3319-3323.

2018

  1. T. Yasuda*, W. Kagawa, T. Ogi, T.A. Kato, T. Suzuki, N. Dohmae, K. Takizawa, Y. Nakazawa, M.D. Genet, M. Saotome, M. Hama, T. Konishi, N.I. Nakajima, M. Hazawa, M. Tomita, M. Koike, K. Noshiro, K. Tomiyama, C. Obara, T. Gotoh, A. Ui, A. Fujimori, F. Nakayama, F. Hanaoka, K. Sugasawa, R. Okayasu, P.A. Jeggo, K. Tajima, Novel function of HATs and HDACs in homologous recombination through acetylation of human RAD52 at double-strand break sites, PLoS Genet, 14 (2018) e1007277.
  2. T. Konishi*, A. Kobayashi, T.F. Tengku Ahmad, J. Wang, Enhanced DNA double strand break repair triggered by microbeam irradiation induced cytoplasmic damage, Journal of Radiation and Cancer Research, 9 (2018) 183-189.
  3. A. Kobayashi, T. Konishi*, Radiation quality effects alteration in COX-2 pathway to trigger radiation-induced bystander response in A549 lung carcinoma cells, J Radiat Res, 59 (2018) 754-759.
  4. S. Kodaira*, Y. Yanagida, Y. Koguchi, H. Kawashima, H. Kitamura, M. Kurano, K. Ogura, Complementary approach for radiation dosimetry with Ag+-activated phosphate glass, Rev. Sci. Instrum., 89 (2018) 116106.
  5. T. Kusumoto*, M. Fromm, P. Cloutier, A.D. Bass, L. Sanche, R. Barillon, T. Yamauchi, Elucidation of the two-step damage formation process of latent tracks in Poly(allyl diglycol carbonate), PADC: Role of secondary low-energy electrons, J. Phys. Chem. C 122 (2018) 21056−21061.
  6. T. Kusumoto*, Z.EL Bitar, S. Okada, P. Gillet, N. Arbor. M. Kanasaki, Y. Mori, K. Oda, A.-M. Nourreddine, H. Kurashige, M. Fromm, P. Cloutier, A.D. Bass, L. Sanche, S. Kodaira, R. Barillon, T. Yamauchi, Radial electron fluence around ion tracks as a new physical parameter for the detection threshold of PADC using Geant4-DNA toolkit, Radiat. Meas., 118 (2018) 50-53.
  7. M. Saotome, K. Saito, T. Yasuda, H. Ohtomo, S. Sugiyama, Y. Nishimura, H. Kurumizaka*, W. Kagawa*, Structural Basis of Homology-Directed DNA Repair Mediated by RAD52. iScience, 3 (2018) 50-62.

 

Institute for Quantum Life Science