Current
Home > Kansai Photon Science Institute > Kansai Photon Science Institute   |   The 45th KPSI Seminar Resolution-exchanged structural modeling and simulations jointly unravel that subunit rolling underlies the mechanism

Kansai Photon Science Institute

Kansai Photon Science Institute   |   The 45th KPSI Seminar Resolution-exchanged structural modeling and simulations jointly unravel that subunit rolling underlies the mechanism

Update:2019年3月8日更新
Display printing page

Kansai Photon Science Institute >> KPSI Seminar >> Resolution-exchanged structural modeling and simulations jointly unravel that subunit rolling underlies the mechanism of programmed ribosomal frameshifting

 

Seminar

The 45th KPSI Seminar

Resolution-exchanged structural modeling and simulations jointly unravel that subunit rolling underlies the mechanism of programmed ribosomal frameshifting

 

Presentor Prof. Lee-Wei Yang
(Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Taiwan)
Place ITBL G201 room (KPSI)
Date 13:15 - (WED) Sep. 26, 2018
Language English
abstract [PDFfile/354KB]

Resolution-exchanged structural modeling and simulations jointly unravel that subunit rolling underlies the mechanism of programmed ribosomal frameshifting 

Prof. Lee-Wei Yang
(Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Taiwan)

abstract

Biological regulation is a manifestation of binding-triggered force controls at the molecular levels. Existing theoretical tools can hardly describe such a control at atomistic details for huge molecular machineries that orchestrate a repertoire of functional motions over long time periods. Here, we leverage linear response theories and resolution-exchanged simulations to study the pseudoknot (PK)-induced force control over programmed ribosomal frameshifting (PRF). Connecting and rationalizing existing structural, single-molecule and mutagenesis data by first principles, we demonstrated how steric hindrance of a stable mRNA structure transiently modifies the conformational dynamics of the ribosome, and subsequently allows tRNA to shift one nucleotide backwards during -1 PRF. Our study provides a temporal and spatial description of PRF with unprecedented mechanistic details to conclude that 30S subunit rolling is the motion that mediates the delicate force control of cis-element unwinding over -1 PRF. The introduced method is also instrumental in studying force-induced controls over other supramolecular machineries.

 

[previous page]
The 44th KPSI Seminar Optical property of a single microdroplet levitated by an electrodynamic ion trap
[next page]
The 46th KPSI Seminar A draft genome of the striped catfish (Pangasianodon hypophthalmus)

Adobe Reader

PDF形式のファイルをご覧いただく場合には、Adobe社が提供するAdobe Readerが必要です。
Adobe Readerをお持ちでない方は、バナーのリンク先からダウンロードしてください。(無料)
Adobe Reader provided by Adobe is required to view PDF format files.