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量子生命科学研究所

シングルセル応答解析チーム_2021年度以前の研究課題、成果

掲載日：2023年4月1日更新
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終了した研究課題

2017年度－2019年度：科研費 基盤研究（B）17H04268「ヒトがん細胞・正常細胞間における放射線誘発双方向シグナリングの研究」小西輝昭（代表）、劉翠華、及川将一、小林亜利紗

2017年度－2019年度：科研費 若手研究（B）17K16496「放射線がん治療におけるバイスタンダー効果の機構解明」小林 亜利紗

2017年度－2020年度：若手研究（A） 17H05093「標的アイソトープ治療に役立てる局所線量分布精密イメージング技術の開発」小平聡（代表）

2016年度－2020年度：科研費 基盤研究（B）16H02971「多元的in situ解析技術によるウラン晩発影響の小児期特性の解明」武田 志乃（代表）、小平聡、小久保年章、小西輝昭、臺野和弘

2016年度－2018年度：挑戦的萌芽研究 16K15586「マイクロビームを用いた細胞核・細胞質照射における防御細胞応答の解析」小西輝昭（代表）

​2021年度の成果

​原著論文​

  1. D. Flint, S. Bright, C. Mcfadden, T. Konishi, D. Ohsawa, B. Turner, S. H. Lin, D. R. Grosshans, H. Chiu, P. Sumazin, S. F. Shaitelman, G. O. Sawakuchi*, Cell lines of the same anatomic site and histologic type show large variability in intrinsic radiosensitivity and relative biological effectiveness to protons and carbon ions. Medical Physics, (in press)
  2. T. Kusumoto*, R. Ogawara, K. Igawa, K. Baba, T. Konishi, Y. Furusawa, S. Kodaira, Scaling Parameter of the Lethal Effect of Mammalian Cells based on Radiation-Induced Oh Radicals: Effectiveness of Direct Action in Radiation Therapy. J Radiat Res, 62 (2021) 86-93.
  3. H. Pan, Y. Song, H. Zhang, Y. Bai, T Konishi, A. Kobayashi, C. Shao*, Y. Pan*, Radiation Engenders Converse Migration and Invasion in colorectal cancer cells through opposite modulation of Anxa2/Akt/Gsk2β pathway., Am J Cancer Res, 11 (2021) 61-78.
  4. A. Uehara *, M. Oikawa, I. Tanaka, H. Ishihara, S. Homma-Takeda, Quantitative Analyses of Urinary Uranium by µ-Pixe, Minerals, 11 (2021) 147.
  5. S. Homma-Takeda*, H. Fujishiro, I. Tanaka, H. Yakumaru, K. Ayama, A. Uehara, M. Oikawa, S. Himeno, H. Ishihara, Single-Cell Imaging for Studies of Renal Uranium Transport and Intracellular Behavior, Minerals, 11 (2021) 191.
  6. T. Yasuda*, K. Takizawa, A. Ui, M. Hama, W. Kagawa, K. Sugasawa, K. Tajima, Human Sirt2 and Sirt3 deacetylases function in DNA homologous recombinational repair. Genes Cells, (2021), https://doi.org/10.1111/gtc.12842.

2020年度の成果

原著論文​​

  1. T. Kusumoto, H. Kitamura, T. Konishi, S. Hojo, S. Kodaira*, Significant Changes in Yields of 7-Hydroxy-Coumain-3-Carboxylic Acid Produced under the Flash Radiotherapy Condition, Rsc Advances, 10(2020)38709
  2. T. Konishi*, D. Yoshihara, M. Maeda, D. Ohsawa, N. Usami, K. Hieda, Enhanced Cell Inactivation and Double-Strand Break Induction in V79 Chinese Hamster Cells by Monochromatic X-rays at Phosphorus K-Shell Absorption Peak, Quantum Beam Science, 4 (2020)38.
  3. J. Wang, A. Kobayashi, D. Ohsawa, M. Oikawa, T. Konishi*, Cytoplasmic Radiation Induced Radio-Adaptive Response in Human Lung Fibroblast Wi-38 Cells, Radiat Res, 194 (2020) 288-297.
  4. M. Suzuki*, Y. Uchihori, H. Kitamura, M. Oikawa, T. Konishi, Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human Fibroblasts, Life (Basel), 10 (2020).
  5. L. Zhu, Q. Chen, L. Zhang, S. Hu, W. Zheng, C. Wang, Y. Bai, Y. Pan, T. Konishi, J. Guan, C. Shao*, Clic4 regulates radioresistance of nasopharyngeal carcinoma by Inos after gamma-rays but not carbon ions irradiation, Am J Cancer Res, 10 (2020) 1400-1415.
  6. R. Ogawara*, T. Kusumoto, A. Kobayashi, T. Konishi, T. Hamano, S. Kodaira, Discrimination of hydroxyl radical yields due to thermal neutrons, fast neutrons, and gamma rays in accelerator-based neutron fields, Radiat Phys Chem, 173 (2020).
  7. R. Ogawara*, T. Kusumoto, T. Konishi, T. Hamano, S. Kodaira, Polyethylene moderator optimized for increasing thermal neutron flux in the Nasbee accelerator-based neutron field, Radiat Meas, 137 (2020).
  8. S. Ishii*, J. Toki, M. Oikawa, D. Ohsawa, T. Konishi, Electrical responses of a carbon nanotube thin-film transistor to Mev proton irradiation in air, Radiation Effects and Defects in Solids, 175 (2020) 440-449.
  9. C. Dong, W. Tu, M. He, J. Fu, A. Kobayashi, T. Konishi*, C. Shao*, Role of Endoplasmic Reticulum and Mitochondrion in Proton Microbeam Radiation-Induced Bystander Effect, Radiat Res, 193 (2020) 63-72.
  10. N. Dukaew, T. Konishi, K. Chairatvit, N. Autsavapromporn, N. Soonthornchareonnon, A. Wongnoppavich*, Enhancement of Radiosensitivity by Eurycomalactone in Human Nsclc Cells Through G(2)/M Cell Cycle Arrest and Delayed DNA Double-Strand Break Repair, Oncol Res, 28 (2020) 161-175.
  11. R. Ogawara*, T. Kusumoto, T. Konishi, T. Hamano, S. Kodaira, Detection of alpha and 7Li particles from 10B(n, α)7Li reactions using a combination of Cr-39 nuclear track detector and potassium hydroxide-ethanol-water solution in accelerator-based neutron fields, Nucl. Instrum. Methods Phys. Res. B., 467 (2020) 9-12.
  12. T. Kusumoto*, R. Barillon, S. Okada, T. Yamauchi, S. Kodaira, Improved criterion of the mechanism for forming latent tracks in poly(allyl diglycol carbonate) based on the number of interactions induced by secondary electrons, Radiat. Meas., 138 (2020) 106445.
  13. T. Kusumoto*, R. Ogawara, N. Ludwig, Q. Raffy, S. Kodaira, Water radiolysis with thermal neutrons, fast neutrons and contamination γ rays in the accelerator based thermal neutron field: Time dependence of hydroxyl radical yields, Radiat. Phys. Chem., 174 (2020) 108978.
  14. T. Kusumoto*, S. Okada, H. Kurashige, K. Kobayashi, M. Fromm, Q, Raffy, N. Ludwig, M. Kanasaki, K. Oda, Y. Honda, S. Tojo, J-E. Groetz, R. Ogawara, S. Kodaira, R. Barillon, T. Yamauchi, Evidence for a critical dose above which damage to carbonate ester bonds in Padc appear after gamma ray and ultra soft X-ray exposures, Radiat. Phys. Chem., 170 (2020) 108628.
  15. S. Kodaira*, T. Kusumoto, H. Kitamura, Y. Yanagida, Y. Koguchi, Characteristics of fluorescent nuclear track detection with Ag+-activated phosphate glas, Radiat. Meas., 132 (2020) 106252.
  16. T. Kusumoto*, Y. Matsuya, K. Baba, R. Ogawara, M.S. Akselrod, J. Harrison, V. Fomenko, T. Kai, M. Ishikawa, S. Hasegawa, S. Kodaira, Verification of dose estimation of Auger electrons emitted from Cu-64 using a combination of Fntd measurements and Monte Carlo simulations, Radiat. Meas., 132 (2020) 106256.
  17. R. Ogawara*, S. Kodaira, M. Suda, T. Hagihara, T. Hamano, Thermal neutron field characteristics in the neutron exposure accelerator system for biological effect experiments (Nasbee) facility, Nucl. Instrum. Meth., A949 (2020) 162724.
  18. S. Homma-Takeda*, A. Uehara, T. Yoshida, C. Numako, O. Sekizawa, K. Nitta, N. Sato, Two-dimensional μXafs for uranium accumulated in kidney of rats exposed to uranyl acetate, Radiat. Phys. Chem., 175 (2020) 108147.
  19. T. Funayama, T. Sakashita, M. Suzuki, Y. Yokota, N. Miyawaki, H. Kashiwagi, T. Satoh and S. Kurashima, An irradiation device for biological targets using focused microbeams of cyclotron-accelerated heavy ions, Nucl. Instrum. Methods Phys. Res. B., 465 (2020), 101-109
  20. M. Suzuki, Z. Soh, H. Yamashita, T. Tsuji and T. Funayama, Targeted central nervous system irradiation of Caenorhabditis elegans induces a limited effect on motility, Biology, 9 (2020), 289
  21. T. Yasuda, T. Funayama, K. Nagata, D. Li, T. Endo, Q. Jia, M. Suzuki, Y. Ishikawa, H. Mitani and S. Oda, Collimated microbeam reveals that the proportion of non-damaged cells in irradiated blastoderm determines the success of development in medaka (Oryzias latipes) Embryos, Biology, 9 (2020), 447

プロシーディング

  1. ​Daisuke Ohsawa,  Alisa Kobayashi,  Teruaki Konishi. "Spatiotemporal dynamics of DNA damage and repair regions from focused proton tracks in human cells after SPICE microbeam irradiation" Proceedings of the 8th Asia Pacific Symposium on Radiation Chemistry (APSRC2020), , 2020-05

2019年度の成果

原著論文​

  1. J. Wang, T. Konishi*, Nuclear factor (erythroid-derived 2)-like 2 antioxidative response mitigates cytoplasmic radiation-induced DNA double-strand breaks, Cancer Sci, 110 (2019) 686-696.
  2. W. Tu, C. Dong, J. Fu, Y. Pan, A. Kobayashi, Y. Furusawa, T. Konishi, C. Shao*, Both irradiated and bystander effects link with DNA repair capacity and the linear energy transfer, Life Sci, 222 (2019) 228-234.
  3. D. Ohsawa*, Y. Furusawa, A. Kobayashi, M. Oikawa, T. Konishi, Analysis of Spice microbeam size using fluorescent nuclear track detector (Fntd), Nucl Instrum Meth B, 453 (2019) 9-14.
  4. T. Konishi*, S. Kodaira, Y. Itakura, D. Ohsawa, S. Homma-Takeda, Imaging Uranium Distribution on Rat Kidney Sections through Detection of Alpha Tracks Using Cr-39 Plastic Nuclear Track Detector, Radiat Prot Dosimetry, 183 (2019) 242-246.
  5. S. Kodaira*, Y. Morokoshi, H.K. Li, T. Konishi, M. Kurano, S. Hasegawa, Evidence of Local Concentration of alpha-Particles from (211)At-Labeled Antibodies in Liver Metastasis Tissue, J Nucl Med, 60 (2019) 497-501.
  6. A. Kobayashi, N. Autsavapromporn, Taf. Ahmad, M. Oikawa, S. Homma-Takeda, Y. Furusawa, J. Wang, T. Konishi*, Bystander Wi-38 Cells Modulate DNA Double-Strand Break Repair in Microbeam-Targeted A549 Cells through Gap Junction Intercellular Communication, Radiat Prot Dosimetry, 183 (2019) 142-146.
  7. W. Hu, L. Zhu, W. Pei, S. Pan, Z. Guo, A. Wu, H. Pei, J. Nie, B. Li, Y. Furusawa, T. Konishi, T.K. Hei, G. Zhou, Overexpression of Ras-Related C3 Botulinum Toxin Substrate 2 Radiosensitizes Melanoma Cells In Vitro and In Vivo, Oxid Med Cell Longev, 2019 (2019) 5254798.
  8. Y. Furusawa*, Y. Matsumoto, R. Hirayama, D. Ohsawa, T. Konishi, Dose-Rate and Cell-Killing Sensitivity of High-Linear Energy Transfer Ion Beam, Radiat Prot Dosimetry, 183 (2019) 219-222.
  9. N. Autsavapromporn*, C. Liu, A. Kobayashi, T. Ahmad, M. Oikawa, N. Dukaew, J. Wang, A. Wongnoppavich, T. Konishi, Emerging Role of Secondary Bystander Effects Induced by Fractionated Proton Microbeam Radiation, Radiat Res, 191 (2019) 211-216.
  10. T. Kusumoto*, Y. Ngono-Ravache, E. Balanzat, C. Galindo, N. Ludwig, Q. Raffy, T, Yamauchi, S. Kodaira, R. Barillon, The role of molecular and radical mobility on the creation of Co2 molecules and Oh groups in Padc irradiated with C and O ions, Polym. Degrad. Stab., 164 (2019) 102-108.
  11. S. Kodaira*, H. Kitamura, M. Kurano, H. Kawashima, E.R. Benton, Contribution to dose in healthy tissue from secondary target fragments in therapeutic proton, He and C beams measured with Cr-39 plastic nuclear track detectors, Sci. Rep., 9 (2019) 3708.
  12. T. Kusumoto*, M. Sakai, A. Yoshida, T. Kambara, Y. Yanagisawa, S. Kodaira, K. Oda, M. Kanasaki, K. Kuraoka, R. Barillon, T. Yamauchi, An update of radial dose distribution theory for the detection threshold of Kapton as a nuclear track detector irradiated with 345 Mev/u U and other heavy ions, Nucl. Instrum. Meth., B460 (2019) 240-243.
  13. T. Kusumoto*, Y. Mori, M. Kanasaki, K. Oda, S. Kodaira, R. Barillon, T. Yamauchi, Drastic decrease of carbonyl group after the loss of ether in Padc exposed to 222 nm UV photons, Radiat. Phys. Chem., 157 (2019) 60-64.
  14. T. Kusumoto*, T. Yamauchi, R. Barillon, Application of radial electron fluence around ion tracks for the description of track response data of polyethylene terephthalate as a polymeric nuclear track detector, Nucl. Instrum. Meth., B461 (2019) 260–266.
  15. R. Ogawara*, M. Suda, T. Hagiwara, S. Kodaira, T. Hamano, Discrimination method for gamma ray doses in neutron fields using an ionization chamber with attenuation filters, Radiat. Prot. Dosim., 183 (2019) 280-2841.
  16. T. Kusumoto*, R. Ogawara, Radiation chemical yield of hydroxyl radicals for accelerator-based boron neutron capture therapy: Dose assessment of 10B(n,α)7Li reaction using coumarin-3-carboxilic solution, Radiat. Res., 191 (2019) 460-465.
  17. S. Homma-Takeda*, C. Numako, K. Kitahara, T. Yoshida, M. Oikawa, Y. Terada, T. Kokubo, Y. Shimada, Phosphorus localization and its involvement in formation of concentrated uranium in the proximal tubules in the kidneys of rats exposed to uranyl acetate, Int. J. Mol. Sci., 20 (2019) 4677-4687.
  18. K. Tajima*, K. Takizawa, T. Yasuda, R. Tsumanuma, S. Okuyama, H. Kumagai, E. Omoto, W. Okumura, Characterization of reemergent anti-B red blood cell antibodies in a patient with recurrent acute myeloid leukemia with Abo-incompatible allogeneic peripheral blood stem cell transplantation. Transfusion, 59 (2019) 3319-3323.

和文誌

  1. 藤巻　秀,  五十嵐　龍治,  高草木　洋一,  安達　基泰,  横谷　明徳,  玉田　太郎,  河野　秀俊,  鹿園　直哉,  今岡　達彦,  赤松　憲,  小西　輝昭,  山田　真希子,  八幡　憲明「最先端の研究開発　量子科学技術研究開発機構　第 4 回　「量子生命科学」の発進」日本原子力学会誌”アトモス”, 61(6), 43 - 47, 2019-06

2018年度の成果

原著論文

  1. T. Yasuda*, W. Kagawa, T. Ogi, T.A. Kato, T. Suzuki, N. Dohmae, K. Takizawa, Y. Nakazawa, M.D. Genet, M. Saotome, M. Hama, T. Konishi, N.I. Nakajima, M. Hazawa, M. Tomita, M. Koike, K. Noshiro, K. Tomiyama, C. Obara, T. Gotoh, A. Ui, A. Fujimori, F. Nakayama, F. Hanaoka, K. Sugasawa, R. Okayasu, P.A. Jeggo, K. Tajima, Novel function of Hats and Hdacs in homologous recombination through acetylation of human Rad52 at double-strand break sites, Plos Genet, 14 (2018) e1007277.
  2. T. Konishi*, A. Kobayashi, T.F. Tengku Ahmad, J. Wang, Enhanced DNA double strand break repair triggered by microbeam irradiation induced cytoplasmic damage, Journal of Radiation and Cancer Research, 9 (2018) 183-189.
  3. A. Kobayashi, T. Konishi*, Radiation quality effects alteration in Cox-2 pathway to trigger radiation-induced bystander response in A549 lung carcinoma cells, J Radiat Res, 59 (2018) 754-759.
  4. S. Kodaira*, Y. Yanagida, Y. Koguchi, H. Kawashima, H. Kitamura, M. Kurano, K. Ogura, Complementary approach for radiation dosimetry with Ag+-activated phosphate glass, Rev. Sci. Instrum., 89 (2018) 116106.
  5. T. Kusumoto*, M. Fromm, P. Cloutier, A.D. Bass, L. Sanche, R. Barillon, T. Yamauchi, Elucidation of the two-step damage formation process of latent tracks in Poly(allyl diglycol carbonate), Padc: Role of secondary low-energy electrons, J. Phys. Chem. C 122 (2018) 21056−21061.
  6. T. Kusumoto*, Z.El Bitar, S. Okada, P. Gillet, N. Arbor. M. Kanasaki, Y. Mori, K. Oda, A.-M. Nourreddine, H. Kurashige, M. Fromm, P. Cloutier, A.D. Bass, L. Sanche, S. Kodaira, R. Barillon, T. Yamauchi, Radial electron fluence around ion tracks as a new physical parameter for the detection threshold of Padc using Geant4-DNA toolkit, Radiat. Meas., 118 (2018) 50-53.
  7. M. Saotome, K. Saito, T. Yasuda, H. Ohtomo, S. Sugiyama, Y. Nishimura, H. Kurumizaka*, W. Kagawa*, Structural Basis of Homology-Directed DNA Repair Mediated by Rad52. Iscience, 3 (2018) 50-62.
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